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I'screen AFLA M1 Milk - AOAC Certified ELISA Kit for the Detection of Aflatoxin M1 in Milk | Gold Standard Diagnostics | YouTubeToText
YouTube Transcript: I'screen AFLA M1 Milk - AOAC Certified ELISA Kit for the Detection of Aflatoxin M1 in Milk
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Video Summary
Summary
Core Theme
The i-ELISA Afla M1 Milk kit is a validated, ready-to-use immunoassay for the quantitative detection of Aflatoxin M1 in milk, offering a reliable and efficient screening method for food safety.
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[Music]
ice cream afla
m1 milk is a sensitive enzyme immunoassay
immunoassay
for the quantitative detection of aflatoxin
aflatoxin
m1 in milk it was developed
by the italian team of researchers at
eurofins techno
and has been available on the market for
20 years
the kit contains all the necessary reagents
reagents
to carry out 96 determinations
there are seven points on the
calibration curve in compliance with iso
14675 2003
all the reagents are provided ready for use
use
the kit is available also in bigger and
smaller versions
with 48 and 192 determinations
the kit contains a manual with
instructions for sample preparation
assay implementation and data calculation
calculation
the ice cream afla m1 milk kit is the
first screening method
that received aoac approval for aflatoxin
aflatoxin
m1 analysis in milk commodities
it has been granted performance tested
method status
for use with raw bovine whole milk skim milk
milk
milk can be analyzed directly or
[Music] water
prepare an assay layout taking into account
account
that two wells are needed for each
standard and sample
prepare an adequate number of wells and reseal
reseal
the unused strips back in the bag with desiccant
desiccant [Music]
[Music]
mark the strips and make a note of the
positions of each standard
and sample add 100 microliters
of each standard in the correspondent wells
wells
and then add one sample one after the other
other [Music]
use single disposable tip for each solution
[Music]
shake the plate gently with a circular
motion for a few seconds
and cover it incubate for 45 minutes
at room temperature in the meantime
prepare an adequate amount of washing
buffer by diluting the concentrated reagent
reagent
provided in the kit for routine analyses
you can prepare a bulk quantity and
store it in the fridge
[Music]
at the end of the incubation pour the
liquid out from the wells
and fill with washing buffer using a
squeeze bottle
pour the liquid out and repeat the
sequence four times
remove the remaining droplets by tapping
the microplate
upside down vigorously against absorbent paper
paper [Music]
add 100 microliters of enzyme conjugate
in each well and incubate the assay for
15 minutes
at room temperature at the end of the
second incubation
repeat the washing procedure four times
tap the plate on the paper to remove the
washing buffer droplets
add 100 microliters of the colorless
developing solution
incubate the assay for 15 minutes at
room temperature [Music]
[Music]
at the end of the incubation add 50
microliters of stop solution
measure the absorbances at 450 nanometers
nanometers
within one hour to calculate
the aflatoxin m1 contamination use the
excel spreadsheet that is available on
our website
the bottom of the product page
contact our customer service team to
learn more about the ice cream afla m1 milk
milk
and other kits for mycotoxin analysis
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