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hey guys quick marker mister basics here
let's talk about admins degradation
method for protein sequencing Edman
degradation is a method used for
sequencing of amino acids in protein in
this method the n-terminal amino acid of
the peptide is first labeled and then
cleaved from the peptide
the label amino acid is then identified
by several techniques like
chromatography or electrophoresis
the labeling of n-terminal amino acid is
done by the reagent called phenol tyo
isocyanate this is also known as Edmonds
reagent
the reaction between Edmonds reagent and
the n-terminal amino acid is done in
mild alkaline conditions
this reaction gives rise to free Nile
Taiyo garb a mild derivative
under heat and
setec conditions this derivative is
cleaved from the parent peptide and
extracted by organic solvents once
extracted it is further
acidified to form PTH amino acid
derivative which is very stable
the PTH amino acid derivative can be
easily identified by chromatography
techniques like HPLC
this reaction can be carried out several
times to find the sequence of the
peptide from the end terminal
the maximum number of amino acids that
can be identified by this technique is
under 30
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