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Edman degradation | Edman Sequencing

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hey guys quick marker mister basics here

let's talk about admins degradation

method for protein sequencing Edman

degradation is a method used for

sequencing of amino acids in protein in

this method the n-terminal amino acid of

the peptide is first labeled and then

cleaved from the peptide

the label amino acid is then identified

by several techniques like

chromatography or electrophoresis

the labeling of n-terminal amino acid is

done by the reagent called phenol tyo

isocyanate this is also known as Edmonds

reagent

the reaction between Edmonds reagent and

the n-terminal amino acid is done in

mild alkaline conditions

this reaction gives rise to free Nile

Taiyo garb a mild derivative

under heat and

setec conditions this derivative is

cleaved from the parent peptide and

extracted by organic solvents once

extracted it is further

acidified to form PTH amino acid

derivative which is very stable

the PTH amino acid derivative can be

easily identified by chromatography

techniques like HPLC

this reaction can be carried out several

times to find the sequence of the

peptide from the end terminal

the maximum number of amino acids that

can be identified by this technique is

under 30

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Edman degradation | Edman Sequencing