YouTube Transcript:
ELISA (Enzyme-linked Immunosorbent Assay)
Skip watching entire videos - get the full transcript, search for keywords, and copy with one click.
Share:
Video Transcript
elisa is short for enzyme-linked immunosorbent sa with this plate-based technique we can detect and quantify soluble substances such as proteins during an elisa the antigen which is the target molecule is immobilized and specifically detected by antibodies which are enzyme-coupled when providing a colorless substrate the enzyme produces a measurable colorful product an antibody is attached to the bottom of the well this antibody is specifically designed for binding a specific protein of interest another soluble antibody is provided the antibody is also specifically covering protein x this antibody is conjugated to an enzyme when we now add a substrate the enzyme can convert it to a colorful product which can be measured later there are many variants of an elisa in the direct elisa the antigen is immobilized on the bottom of the plate and the enzyme-linked antibody directly binds to the antigen in the indirect elisa a primary antibody binds our antigen and then a secondary antibody binds the primary antibody the secondary antibody is enzyme linked the most common type is the sandwich elisa a primary antibody is immobilized and captures the antigen of a solution and another primary antibody specifically binds the same antigen but all three elisa's have one thing in common the last antibody binding is always conjugated to the enzyme which confirms binding here we provide an example of an elisa for this sandwich elisa we have two wells both coated with primary antibody specific for protein x on the left we provide a sample containing our protein on the right we have the control without the target protein our antigen in orange will be immobilized a washing step will discard everything else now we provide the enzyme-linked antibody it will bind our antigen in the left well and remains bound after washing whereas the antibody is discarded in the control in the last step a colorless substrate is given since the enzyme in most cases horseradish peroxidase is exclusively present in the left well it produces a measurable colorful product here this can be investigated and also quantified using spectophotometry the control remains colorless since substrate is not converted here if you want to have a closer look into the direct or the indirect elisa you may click one of these videos here
Share:
Paste YouTube URL
Enter any YouTube video link to get the full transcript
Transcript Extraction Form
How It Works
Copy YouTube Link
Grab any YouTube video URL from your browser
Paste & Extract
Paste the URL and we'll fetch the transcript
Use the Text
Search, copy, or save the transcript
Why you need YouTube Transcript?
Extract value from videos without watching every second - save time and work smarter
YouTube videos contain valuable information for learning and entertainment, but watching entire videos is time-consuming. This transcript tool helps you quickly access, search, and repurpose video content in text format.
For Note Takers
- Copy text directly into your study notes
- Get podcast transcripts for better retention
- Translate content to your native language
For Content Creators
- Create blog posts from video content
- Extract quotes for social media posts
- Add SEO-rich descriptions to videos
With AI Tools
- Generate concise summaries instantly
- Create quiz questions from content
- Extract key information automatically
Creative Ways to Use YouTube Transcripts
For Learning & Research
- Generate study guides from educational videos
- Extract key points from lectures and tutorials
- Ask AI tools specific questions about video content
For Content Creation
- Create engaging infographics from video content
- Extract quotes for newsletters and email campaigns
- Create shareable memes using memorable quotes
Power Up with AI Integration
Combine YouTube transcripts with AI tools like ChatGPT for powerful content analysis and creation:
Frequently Asked Questions
Is this tool really free?
Yes! YouTubeToText is completely free. No hidden fees, no registration needed, and no credit card required.
Can I translate the transcript to other languages?
Absolutely! You can translate subtitles to over 125 languages. After generating the transcript, simply select your desired language from the options.
Is there a limit to video length?
Nope, you can transcribe videos of any length - from short clips to multi-hour lectures.
How do I use the transcript with AI tools?
Simply use the one-click copy button to copy the transcript, then paste it into ChatGPT or your favorite AI tool. Ask the AI to summarize content, extract key points, or create notes.
Timestamp Navigation
Soon you'll be able to click any part of the transcript to jump to that exact moment in the video.
Have a feature suggestion? Let me know!
Get Our Chrome Extension
Get transcripts instantly without leaving YouTube. Install our Chrome extension for one-click access to any video's transcript directly on the watch page.