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How to Make an SDS-PAGE gel
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today I'll be showing you how to cast an SDS page gel here is is the apparatus for casting the Jael there are four different parts to this jaw apparatus uh first of all you have two glass plates uh you have a short plate and then you have a toll plate then you also have a a casting frame and a casting stand what you do here right now is you want to place the short plate in front of the toe plate like that and then put it into the casting frame after you putting the casting frame you secure it by clamping it and then you want to check that the button is flat when everything is done then you can put it on the casting stand okay after we have assembled the jaw apparatus what we need to do right now is we will take the comb and we'll place it in between the glass plates like that and we'll take a marker and we'll make a mark approximately 1 cm beneath below the comb right there we take the comb out what we want to do right now is we want to add a little bit of water to the glass plate to see whether that leak heat or not so as you can see right now uh the apparatus is not leaky so therefore we'll draw out the water using a piece of filter paper okay now we'll be casting the resolving Jael so so I have added everything on the recipe except for Tate Tate is going to initialize their plation reaction so after you add a teammate you should cast the jaw right away so now I have just added teammate to my Sol ution so we want to pour the gel right away we want to pour the gel till the gel is at the same level as the Mark it's okay if you introduce any air bubbles right now we'll fix that later so we'll pour the gel to the mark and then we'll remove the bubbles by adding a layer of isop perano on top of the gel like that so after the resolving jaw has polymerized we will try out the isopo using the filter paper [Music] again after we draw the isopo we can cast the stacking gel so right now I'm going to add team it to the stocking gel so we'll fill up the rest of the plate with stocking gel we'll fill it all the way to the top like that right now you don't want to introduce any air bubble because you're just going to get trapped and then we'll put in the comb and that's how you cast an SDS gel [Music]
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