YouTube Transcript:
How to Make an SDS-PAGE gel

today I'll be showing you how to cast an
SDS page
gel here is is the apparatus for casting
the Jael there are four different parts
to this jaw apparatus uh first of all
you have two glass plates uh you have a
short plate and then you have a toll
plate then you also have a a casting
frame and a casting stand what you do
here right now is you want to place the
short
plate in front of the toe plate like
that and then put it into the casting
frame after you putting the casting
frame you secure it by clamping it and
then you want to check that the button
is flat when everything is
done then you can put it on the casting
stand okay after we have assembled the
jaw apparatus what we need to do right
now is we will take the comb and we'll
place it in between the glass plates
like that and we'll take a marker and
we'll make a mark approximately 1 cm
beneath below the comb right
there we take the comb out what we want
to do right now is we want to add a
little bit of water to the glass plate
to see whether that leak heat or not
so as you can see right now uh the
apparatus is not
leaky so therefore we'll draw out the
water using a piece of filter
paper okay now we'll be casting the
resolving Jael so so I have added
everything on the recipe except for Tate
Tate is going to initialize their
plation reaction so after you add a
teammate you should cast the jaw right
away so now I have just added
teammate to my Sol ution so we want to
pour the gel right
away we want to pour the gel till the
gel is at the same level as the
Mark it's okay if you introduce any air
bubbles right now we'll fix that
later
so we'll pour the gel to the
mark and then we'll remove the bubbles
by adding a layer of isop perano on top
of the gel like
that so after the resolving jaw has
polymerized we will try out the isopo
using the filter paper
[Music]
again after we draw the isopo we can
cast the stacking
gel so right now I'm going to add team
it to the stocking
gel so we'll fill up the rest of the
plate with stocking
gel we'll fill it all the way to the
top like that right now you don't want
to introduce any air bubble because
you're just going to get
trapped and then we'll put in the comb
and that's how you cast an SDS gel
[Music]