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Hematology: Performing and Interpreting QC on the Sysmex XN-550 Automated CBC Analyzer | Medical Lab Lady Gill | YouTubeToText
YouTube Transcript: Hematology: Performing and Interpreting QC on the Sysmex XN-550 Automated CBC Analyzer
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Video Summary
Summary
Core Theme
This content provides a step-by-step guide on how to operate the XN550 hematology analyzer, focusing on the initial setup, running quality control (QC) using the drawer sampler, and interpreting QC results.
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everyone today we're using the xn550
and uh what we're gonna do is just show
you how to
turn it on and start using it uh we'll
be using manual mode today
for the specimens but we're gonna be
using the
drawer sampler for our qc so
first things first we got to turn this
puppy on so
it is on in the back um the backed switch
switch
we're going to turn the green light on
you're going to notice
right away that over over on the right
the manual sample door you've got the
red light
the touch screen immediately comes on starts
starts
turning green and that
that indicates that there is a
connection there
that barcode reader is flashing green
all right
i've got my qc out it should come to
room temperature
for 15 minutes before you start running
it we use three levels of qc
and this is the
xn xn check
qc level one is the red
and level three is the black
okay and how these are um
indicated by level one level two level
three level one is a low
level two is a medium
range and then level three is your high
abnormal range so the low and the high would
would
make sure that the range of which you are
are
analyzing specimens is actually accurate
um we've talked in other videos about
the analytical measurable range
and the clinical reportable range the
analytical measurable range is what your
analyzer can
do without the aid of dilution
and detect
from the low range to the higher range
and that includes the normal range or
reference range as well
whereas the clinical reportable range is
what your facility has decided
is able to be reported out
with accuracy so that mainly extends on
the high
side of what analysis
will be acceptable on the high
side outside of the cl outside of the
analytical measurable range
okay so while i'm waiting for the
analyzer to continue
to warm up i am inverting my qc
okay i've i'm making sure that before i
did this the caps are on
very tightly and i'm just
inverting them up and down you should do
this about 20 times
before you put it on so it's a nice
rocking motion
inverting motion that you can do yourself
yourself
manually please don't use a rocker an
automated rocker
sysmex has sysmex has recommended not
to use a rocker and
i'm going to pause this because it's
going to take a little bit for it to
pull up and i don't want you sitting here
here
watching while it's just warming up so
i'll come back
all right so at this point everything is
ready to go
we have this beautiful green light down
at the bottom of the screen
all right now it's asking us for our
passwords you can find the password
in your lab reports you can also find
the password and the
supplied quick guide manual from the
um the company says mix
so this is a touch screen you can use
the touch screen you can also
use the um the keyboard
i'm going to use the keyboard at this
moment to show you that that works as well
well
you can't use it with all
you can absolutely try so we also have a
mouse as well
all right so what's happening now is we
are in
it's bringing up the the main menu of
what it is that you uh can do on this analyzer
analyzer
so the first thing we are going to do is
uh we are going to do the qc
so first things first you want to make
sure that in
this bottom right hand corner of the
touch screen it's in sampler mode
that means we're going to use the drawer okay
okay
for your specimen analysis you're going
to make sure that you turn
that to manual but right now we need it
in sampler mode we also need that
beautiful green light on that left hand
bottom of your touch screen to show that
we are in ready mode
so before you get started on anything
you always want to make sure that
your adapter to the little holder
the metal piece in the right position it
looks great
so um you can either take out
this and load it or put it
in keep it in here and then load it so i
have made sure that i have inverted
um lovingly inverted my qc
uh probably more than 20 times at this
point uh the analyzer is going to invert
it as well there's an arm
in there which you can barely see
because of the glare
see that black piece right there uh in
the glare
of the tube the qc tube that's where the
arm is it comes
and it picks these up and it's going to
invert them
so i'm going to do level one in position one
one
okay this is the way it always is 10 is
at the back
one is in the front all right we've got level
level
two gonna load that now and number two
and level three is in
number three the um the question you may
have is
okay well if that's the case
if we know that there's bar codes and we
know that this is the automated side
why do we care because
it will automatically read the barcode and
and
um that sort of thing but the thing is
is that you want to indicate
where the first tube is okay
looking at this picture you even see
that your rack needs to be
in like this with 10 in the back one in
the front
if you stuck the uh qc
in um position six seven or eight you
know something back here
it's going to go and it's going to check
every single position that's just a
waste of time
all right so the reason that we go and
do this is that we're showing
the analyzer where to start we see that it's
it's
in cbc diff mode that's perfect
this is whole blood qc meaning that it's
going to test all your parameters
as if it's running a patient whole blood specimen
specimen
so that being said if you had a body
fluid you would need to make sure to get
the appropriate qc for that type of analysis
analysis
so we already pressed i mean we'll press
it again just to show you how it works
press ok all right and now
we're ready to go ahead and press the
start button
done that's that blue button right here
all right you see that that drawer
starts to
it's going to the front it's going to analyze
analyze
and see oh my gosh i wish the glare
wasn't there
it's saying yes it is in position one mode
mode
it picked up the first qc look at that
now it's going to go back
the cap is on good thing [Laughter]
[Laughter]
that qc would be all over the place
now it's going to take it to that
sample analysis location that would be
the manual one that we'll do later for specimens
specimens
it's back reading the barcode now you
saw the barcode
wait pull it out once it's done
with the aspiration pulling out from the
qc tube it's back
out the arm's picking it up again
taking it back i really apologize for
the glare and it's putting it back in
that spot
i'm gonna invert it just like it did
over to that manual load area
put it in there and it's going to read
the barcode it just went back in
it's back in there aspirating the door
is closed
so that nothing gets in there it's
protecting you and there it is it's out again
arm picks it up and puts it back
all right so you've seen that twice i
don't think you need to watch that anymore
anymore
so what we're going to look for is
where can we see this at this point
where can we see
what the results look like if we have
any so far
so we have the printer i'm going to turn
it on
okay the on button's right here in the
front all right
once we have results it will come out
there see it's turning on um if
if you don't have it turned on obviously
it won't do that so
here we could go to our work list
we can see what's running right now
there is
no specimen running so that's why you
don't see anything
here we go to the qc
this is what we find out all right
so these in red were back in 2019
that's not what we're doing right now we
are doing the one
um the ones down here okay um
so we've got uh a really weird date there
there
um that's the expiration date that's not
the date that we have right now the
analysis date is right here
so it's showing us what time it is um
if it's expired or not it's not expired
so that's great
we can go and take a look at it and see
all right is it in um
so we can go along here and see
um what by arrowing down
the parameters that were measured so
this is all
of an automated cbc right and then we
go down we've got the diff the automated diff
diff
we've got a little bit of divi deviation
okay but everything is within this blue shaded
shaded
area so that's your 2sd
range there okay so everything looks
gorgeous oh my goodness that's perfect right
right
so let's go
back to the qc file we could check
you can um you can see that at the top
of your screen
i move my tripod a little bit
we saw everything look great okay the
red line means that's today
that's our specific run all right it's
been run other times before because we
had to set up
the qc the instrument was updated
we had to run qc to set the new lot okay
so looking at all these they look great
all right going up
to the top everything looks wonderful
so you can also see qc in a different way
way
so i'm in the third level here
level three you see i'm going to go up to
to
all right and it shows the results
this way okay you see it's qc
all right you see the number there is
whole blood
it's the time time and date all right
and you see how it fares
with the normal range and you know what
the units are
all of that you can graph this look at that
that
so you see the scatter plot here
the histogram of the reds and the platelets
platelets
um are there any flags
no that's great and this comes into play
when you
are in specimen analysis
okay we're just looking through but you
don't you don't need to go really past
that so
um you graph it out it looks like that
and then
your main here uh there is
a qc radar that you can do
i prefer the levy jennings which i
already showed you so
all right so since our qc did pass um
we're going to go ahead and we're going
to run some specimens
all right so i'm going to end this video and
and
we're gonna do we're gonna do a regular
um specimen that you would do like in class
class
all right so i'll see you next time
please make sure to subscribe if you
like this video
and i'll catch you next time have a
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