This content details the procedure for performing a Total Leukocyte Count (TLC) using a Neubauer chamber, including the necessary materials, step-by-step instructions, calculation methods, and explanations of related physiological and pathological conditions.
Mind Map
Click to expand
Click to explore the full interactive mind map • Zoom, pan, and navigate
my name is anish kulkarni i'm a first
year mbbs student from hbtmc and cooper
hospital today i'll be performing uh the
total leukocyte count experiment so
first we'll check the apparatus for the
experiment and then we'll i'll tell you
the basic things we require are spirit
some cotton
a lancet
other than that we require turks fluid
or the white blood cell diluting fluid
the nubus chamber
this is the improved nua so the first
thing we need to do is sterilize your
hand to make sure that you do not get
any infections
be sure to take the ring finger for for
any pricking
procedure as it is not connected to the bursa
i use the pipette and this uh suck
suck
to take the blood inside the pipet up
if any excess blood enters
the pivot then use the palm of your hand
and just tap the pipette till the blood
is out of the pipette
then clean off your hand and the tip of
then add the turks fluid up till the 11
and then uh use the pipette and suck the
diluting fluid up till the 11 mark which
okay now further dial hitting fluid
until the eleventh point then pull with
your arms
then uh
uh
take a drop here and then drop it in the
new pass chamber it will slide across
the whole numbers chamber due to the
now let the slide settle for one to two minutes
minutes
after you are done charging the new boss
chamber and you are done waiting for one
to two minutes uh
place the nubus chamber in on the microscope
microscope
as like this
then to count use a 10x microscope lens
to count place this at 10. [Music]
[Music]
so this is the diagram for the nubus
chamber so here here here and here we
count the white blood cells so start
from here uh and uh when you see in the
microscope whatever you see however many
white blood cells you see start writing
it here
and then complete this whole
chamber dimension so the dimensions of
the nubus chamber are 1 mm of length and
bread and 0.1 mm of height so the total
volume comes to 0.1 mm per so this is
what the completed nuvar chamber will uh
diagram will look like with each square
having some number of white blood cells
now i'll tell how the dialogue factor of
20 is achieved so first you take a 0.5
ml worth of blood and then you add 11 ml
worth of diluting factor then you
discard some amount of the
the
fluid in the pipette in fluid so if you
multiply it by 2 then 1 ml of the blood
contains 20 ml of the diluting fluid
so now coming to the calculation part
so the volume of each universe chamber is
is
1 into 1 into 0.1
0.1 mm is the height 1 mm 1 mm is the
length and 1 mm is the breadth which
comes down to 0.1 mm cube now this is
for one box so for four boxes it is
going to be into four which is equal to
0.4 mm cube
of volume now
now
after calculating the total number of
wbcs from these boxes it comes down to 198
198
so if 0.4 mm cube
has 198 wbcs
so 1 mm cube
let's take this as x
so x is going to come out as 198 divided
by 0.4 now
now
this this part won't give us the final
answer because we'll have to multiply
this by the diluting factor as well
which is which is explained before
and hence after calculating this the
total number of rbcs will come down as
9900 per
so the first question is what is the
composition of wbc diluting fluid and
what are the functions of each constrictor
constrictor
so the wbc dilating fluid is also known
as turks fluid it contains glacial
acetic acid
which destroys the membranes of our bc
wbc and platelets the second thing it
contains its methyl violet which colors
the fluid and also stains the nuclei of
the white blood cells and third is
distilled water which is used as a
solvent coming to the second question in
his definition of leukocytosis
leukopenia and leukemia
leukemia
so the leukocytosis is increase in a wbc
number leukopenia is decreasing wbc
number and leukemia is excess amount of
white blood cells in the peripheral
blood system
okay the normal range of white blood
cells is four thousand to eleven
thousand uh so in leukocytosis uh the
range uh the number increases above
eleven thousand that is why it is
increasing white blood cells number that
is leukocyte second is leukopenia in it
uh the white blessing decreases below
four thousand and third is leukemia in
which the white blood cell count
increases well above fifty thousand
question three is enumerate the causes
of physiological leukocytosis uh the
physiological causes are one pregnancy
or menstruation for women second is
muscular exercise
third is diurnal variation and the fourth
fourth
is puberty
pathological causes of leukocytosis
pathological causes of leukocytes are
acute and chronic infection the
the
allergic reactions and leukemia
the pyogenic bacterial infection is the
most common cause of increase in white
leukocytosis pathological causes of
leukopenia typhoid fever sulphur drugs
and irradiation
and then
where are the wbcs produced in the body
the wbcs are produced in the bone marrow
what is the function of this bead in the
bulb the first function is it aids in
mixing of the blood with diluent the
secondly it helps in identifying the
pipette as you can see the blade is
white in color so it is for the white
blood cells and third it tells whether
the pipette is dry or not in a dry pet
the bead will uh
go around freely but in a wet pipette it
will stick to the surface and will not
uh move around freely
so now we'll discuss the possible
sources of error and how to minimize
them one of the sources of error is
if the pipet is wet
clean and dry the pipette
okay and second source of error is if
blood is
sucked beyond the 0.5 mark and tap it
against your palm to go below the 0.5 mark
mark
and if there is formation of air bubbles
while sucking off blood well pick the
hand carefully and then suck the bell
and then if if there is sedimentation of
rbc's in the bulb roll the pipette in
your palms
to mix the blood and
fluid well and why do we need to discard
the first few drops of rbc because they
so that's it for the white blood count
Click on any text or timestamp to jump to that moment in the video
Share:
Most transcripts ready in under 5 seconds
One-Click Copy125+ LanguagesSearch ContentJump to Timestamps
Paste YouTube URL
Enter any YouTube video link to get the full transcript
Transcript Extraction Form
Most transcripts ready in under 5 seconds
Get Our Chrome Extension
Get transcripts instantly without leaving YouTube. Install our Chrome extension for one-click access to any video's transcript directly on the watch page.
