0:03 [Music]
0:04 ice cream afla
0:07 m1 milk is a sensitive enzyme immunoassay
0:08 immunoassay
0:10 for the quantitative detection of aflatoxin
0:11 aflatoxin
0:14 m1 in milk it was developed
0:16 by the italian team of researchers at
0:18 eurofins techno
0:20 and has been available on the market for
0:23 20 years
0:25 the kit contains all the necessary reagents
0:26 reagents
0:29 to carry out 96 determinations
0:31 there are seven points on the
0:35 calibration curve in compliance with iso
0:38 14675 2003
0:40 all the reagents are provided ready for use
0:42 use
0:44 the kit is available also in bigger and
0:45 smaller versions
0:49 with 48 and 192 determinations
0:51 the kit contains a manual with
0:54 instructions for sample preparation
0:56 assay implementation and data calculation
0:58 calculation
1:01 the ice cream afla m1 milk kit is the
1:03 first screening method
1:05 that received aoac approval for aflatoxin
1:06 aflatoxin
1:09 m1 analysis in milk commodities
1:11 it has been granted performance tested
1:13 method status
1:16 for use with raw bovine whole milk skim milk
1:16 milk
1:22 milk can be analyzed directly or
1:38 [Music] water
1:45 prepare an assay layout taking into account
1:46 account
1:48 that two wells are needed for each
1:54 standard and sample
1:56 prepare an adequate number of wells and reseal
1:57 reseal
2:00 the unused strips back in the bag with desiccant
2:00 desiccant [Music]
2:01 [Music]
2:04 mark the strips and make a note of the
2:05 positions of each standard
2:09 and sample add 100 microliters
2:11 of each standard in the correspondent wells
2:12 wells
2:14 and then add one sample one after the other
2:16 other [Music]
2:23 use single disposable tip for each solution
2:28 [Music]
2:30 shake the plate gently with a circular
2:32 motion for a few seconds
2:35 and cover it incubate for 45 minutes
2:38 at room temperature in the meantime
2:40 prepare an adequate amount of washing
2:43 buffer by diluting the concentrated reagent
2:44 reagent
2:47 provided in the kit for routine analyses
2:50 you can prepare a bulk quantity and
2:51 store it in the fridge
2:59 [Music]
3:01 at the end of the incubation pour the
3:03 liquid out from the wells
3:05 and fill with washing buffer using a
3:06 squeeze bottle
3:08 pour the liquid out and repeat the
3:11 sequence four times
3:13 remove the remaining droplets by tapping
3:14 the microplate
3:17 upside down vigorously against absorbent paper
3:19 paper [Music]
3:27 add 100 microliters of enzyme conjugate
3:29 in each well and incubate the assay for
3:30 15 minutes
3:33 at room temperature at the end of the
3:35 second incubation
3:38 repeat the washing procedure four times
3:40 tap the plate on the paper to remove the
3:47 washing buffer droplets
3:50 add 100 microliters of the colorless
3:51 developing solution
4:01 incubate the assay for 15 minutes at
4:03 room temperature [Music]
4:06 [Music]
4:08 at the end of the incubation add 50
4:10 microliters of stop solution
4:23 measure the absorbances at 450 nanometers
4:24 nanometers
4:28 within one hour to calculate
4:31 the aflatoxin m1 contamination use the
4:33 excel spreadsheet that is available on
4:34 our website
4:43 the bottom of the product page
4:45 contact our customer service team to
4:48 learn more about the ice cream afla m1 milk
4:48 milk
4:51 and other kits for mycotoxin analysis
