0:08 in the cytosol of an
0:11 eukaryotic cell two types of erf
0:16 are present they are erf1
0:19 and erf3
0:23 the erf stands for eukaryotic release factor
0:25 factor
0:30 the erf3 is a gtp binding protein
0:34 when a ats ribosome having a newly formed
0:35 formed
0:38 completed protein chain reaches a stop codon
0:39 codon
0:42 like uaa or
0:46 uga or uag
0:50 the erf1 along with erf3 dot
0:54 gtp enters the ribosomal complex
0:59 probably at or near the a side
1:03 and the erf1 recognize this top corner
1:06 directly the other name
1:10 of erf3.gtp
1:14 is erf3 gt ph
1:18 the recognition of stop codon by erf-1
1:21 leads to erf3 bound gtp
1:26 hydrolysis to produce a gdp
1:30 and a pi here the hydrolysis step
1:33 acts as a proof reading step
1:37 because it ensures that the previous step
1:38 step
1:41 means the recognition of a stop codon
1:45 by erf 1 has occurred correctly
1:49 and then only it allows subsequent step
1:54 or further steps to proceed after the
1:58 ensurement given by the hydrolysis
2:01 that the erf1 has correctly recognized
2:05 a stop codon the peptidyl trna
2:08 present in the p site of a ribosomal complex
2:09 complex
2:13 is added with a h2o molecule
2:18 in presence of peptidyl transferase
2:21 due to this addition reaction the newly
2:23 formed completed protein chain
2:27 separates from the trna and
2:30 released into the cytosol then
2:33 additional release factors promote
2:38 the dissociation of ats ribosome
2:41 as a result the 40s and
2:45 60s ribosomal subunits mrna
2:49 trnas erf1
2:53 dot gdp are freed to carry out
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